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cfse dye  (Elabscience Biotechnology)


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    Elabscience Biotechnology cfse dye
    Cfse Dye, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cfse+dye/CFSE+Cell+Division+Tracker+Kit/pm41288742-137-15-18
    Average 94 stars, based on 12 article reviews
    cfse dye - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Labeling:

    Article Title: CpG ODN Activates TLR9 and Upregulates TLR3 via the p38 MAPK-ATF3 Signaling Axis to Synergistically Enhance Dendritic Cell Vaccine Efficacy
    Article Snippet: .. CFSE Labeling of CD8+ T Cells Mouse splenic lymphocytes or human peripheral blood-derived CD8+ T cells were pre-labeled with CFSE dye (E-CK-A345, Elabscience, Wuhan, China) at a concentration of 5 uM for 20 min at 37 ◦C protected from light. ..

    Concentration Assay:

    Article Title: CpG ODN Activates TLR9 and Upregulates TLR3 via the p38 MAPK-ATF3 Signaling Axis to Synergistically Enhance Dendritic Cell Vaccine Efficacy
    Article Snippet: .. CFSE Labeling of CD8+ T Cells Mouse splenic lymphocytes or human peripheral blood-derived CD8+ T cells were pre-labeled with CFSE dye (E-CK-A345, Elabscience, Wuhan, China) at a concentration of 5 uM for 20 min at 37 ◦C protected from light. ..

    Article Title: CpG ODN Activates TLR9 and Upregulates TLR3 via the p38 MAPK-ATF3 Signaling Axis to Synergistically Enhance Dendritic Cell Vaccine Efficacy
    Article Snippet: .. Mouse splenic lymphocytes or human peripheral blood-derived CD8 + T cells were pre-labeled with CFSE dye (E-CK-A345, Elabscience, Wuhan, China) at a concentration of 5 uM for 20 min at 37 °C protected from light. ..

    Activation Assay:

    Article Title: Histone lactylation drives METTL3 upregulation-mediated RNA m6A modification of CCT2 to hinder CD8 + T cell survival in gastric cancer.
    Article Snippet: .. 333 2.16 Detection of the proliferation and activation of CD8+ T 334 cells 335 The CFSE dye (E-CK-A345, Elabscience, China), APC Anti336 Human IFN - γ Antibody (E-AB-F1196E, Elabscience, China), PerCP 337 anti-human TNF - α Antibody (502924, Biolegend, USA), and PE/Cy7 338 anti-human Granzyme B Antibody (372214, Biolegend, USA) were 339 utilized to label CD8+T cells from different treatment groups. ..



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    Combined treatment with LAG3pep-2 and anti-programmed death-ligand 1 (anti-PD-L1) antibody restores T cell activity against tumor cells. (A) Experimental schemes. CD8+ T cells were isolated from the spleen of MC38 tumor-bearing mice and incubated for 48 h with anti-CD3/CD28 beads (activation) and interleukin-2 (IL-2)/interleukin-15 (IL-15) (proliferation). The activated T cells were co-cultured with MC38 cells in the absence or presence of LAG3pep-2 and anti-mouse PD-L1 antibody alone or in combination. Created with BioRender.com . (B) Activated CD8+ T cells were stained with <t>carboxyfluorescein</t> <t>succinimidyl</t> ester <t>(CFSE)</t> dye and co-cultured with tumor cells for 24 h. The population of CD3+/CFSE− cells was measured. (C to E) After co-culturing for 24 h, the culture medium was collected, and the percentage of cell death (lactate dehydrogenase [LDH] release) (C) and the concentrations of interferon-γ (IFN-γ) (D) and granzyme B (E) were measured. Data are presented as the mean ± SD of 3 independent experiments. * P < 0.05; ** P < 0.01; *** P < 0.001; ns, not significant by one-way analysis of variance (ANOVA).
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    Image Search Results


    Combined treatment with LAG3pep-2 and anti-programmed death-ligand 1 (anti-PD-L1) antibody restores T cell activity against tumor cells. (A) Experimental schemes. CD8+ T cells were isolated from the spleen of MC38 tumor-bearing mice and incubated for 48 h with anti-CD3/CD28 beads (activation) and interleukin-2 (IL-2)/interleukin-15 (IL-15) (proliferation). The activated T cells were co-cultured with MC38 cells in the absence or presence of LAG3pep-2 and anti-mouse PD-L1 antibody alone or in combination. Created with BioRender.com . (B) Activated CD8+ T cells were stained with carboxyfluorescein succinimidyl ester (CFSE) dye and co-cultured with tumor cells for 24 h. The population of CD3+/CFSE− cells was measured. (C to E) After co-culturing for 24 h, the culture medium was collected, and the percentage of cell death (lactate dehydrogenase [LDH] release) (C) and the concentrations of interferon-γ (IFN-γ) (D) and granzyme B (E) were measured. Data are presented as the mean ± SD of 3 independent experiments. * P < 0.05; ** P < 0.01; *** P < 0.001; ns, not significant by one-way analysis of variance (ANOVA).

    Journal: Biomaterials Research

    Article Title: A Peptide Inhibitor of Lymphocyte Activation Gene-3 Interaction with Fibrinogen-like Protein 1 Synergizes with Programmed Death-Ligand 1 Blockade to Restore T Cell Activity and Inhibit Tumor Growth

    doi: 10.34133/bmr.0364

    Figure Lengend Snippet: Combined treatment with LAG3pep-2 and anti-programmed death-ligand 1 (anti-PD-L1) antibody restores T cell activity against tumor cells. (A) Experimental schemes. CD8+ T cells were isolated from the spleen of MC38 tumor-bearing mice and incubated for 48 h with anti-CD3/CD28 beads (activation) and interleukin-2 (IL-2)/interleukin-15 (IL-15) (proliferation). The activated T cells were co-cultured with MC38 cells in the absence or presence of LAG3pep-2 and anti-mouse PD-L1 antibody alone or in combination. Created with BioRender.com . (B) Activated CD8+ T cells were stained with carboxyfluorescein succinimidyl ester (CFSE) dye and co-cultured with tumor cells for 24 h. The population of CD3+/CFSE− cells was measured. (C to E) After co-culturing for 24 h, the culture medium was collected, and the percentage of cell death (lactate dehydrogenase [LDH] release) (C) and the concentrations of interferon-γ (IFN-γ) (D) and granzyme B (E) were measured. Data are presented as the mean ± SD of 3 independent experiments. * P < 0.05; ** P < 0.01; *** P < 0.001; ns, not significant by one-way analysis of variance (ANOVA).

    Article Snippet: The CD8+ T cells were stained with 5 μM of carboxyfluorescein succinimidyl ester (CFSE) dye (Thermo Fisher Scientific) in an incubator at 37 °C for 20 min and then co-cultured with tumor cells for 24 h. Next, cell proliferation was examined by counting the CD3+ T cells using a flow cytometer.

    Techniques: Activity Assay, Isolation, Incubation, Activation Assay, Cell Culture, Staining